Composite
Part:BBa_K415148:Experience
Designed by: Jason Stevens Group: iGEM10_MIT (2010-10-24)
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Applications of BBa_K415148
Assessing p8-Jun incorporation into phage coat
We transformed XL1-Blue cells with BBa_K415149, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody.
There was no signal from the blot, indicating that inducing the part may be necessary for incorporation into the phage coat.
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UNIQ1ac6424fd9385260-partinfo-00000000-QINU UNIQ1ac6424fd9385260-partinfo-00000001-QINU